Spexin as a Satiety Factor in Mouse via Regulatory Actions Within the Hypothalamus

Abstract Spexin (SPX) is a pleiotropic peptide with highly conserved protein sequence from fish to mammals and its biological actions are mediated by GalR2/GalR3 receptors expressed in target tissues. Recently, SPX was found be novel satiety factor models but whether the has similar function still unknown. Using mouse as model for mammalian species, functional role of feeding control mechanisms involved were investigated. After food intake, serum could up-regulated mice parallel elevations transcript expression tissue content glandular stomach not other tissues examined. As revealed immunostaining, intake also intensified signals different cell types within gastric mucosa stomach. Furthermore, IP injection effective reducing consumption drops NPY, AgRP, NPY type 5 receptor (NPY5R) ghrelin (GHSR) hypothalamus, these inhibitory effects blocked GalR3 GalR2 antagonism. In agreement central SPX, inhibition on hypothalamic NPY5R GHSR observed ICV SPX. same study, rises leptin (LepR) melanocortin 4 (MC4R) hypothalamus. These findings, taken together, suggest that well-conserved mouse. Probably, can induce production contribute postprandial rise circulation. Through activation, this signal act hypothalamus trigger feedback differential modulation regulators (NPY & AgRP) their (NPY5R, GHSR, LepR MC4R) circuitry brain.